Sterlization

Sterilization: - It is the process of inactivating or remove all the microbes and spores from the surface areas. Sterilization procedures provide the foundation for all bacteriological or sterile techniques and this process have common place in our everyday life.
For example: - In a hospital virtually everything must be sterilized.

The objectives of sterilization may be summarized as follows: -

1. To avoid the contamination of the product.
2. To permit only desired strain to be present in the vessel.
3. To ensure the safety of the product.
4. To prevent the deterioration of the products.

The sterilization may be achieved by heating, radiation and filtration process.

Pasteurization: - It is the process of mild heating of chemical of milk for removing the pathogens which are harmful for human beings.
For 72*c , 15 seconds sterilization.
For 140*c , less than 1 seconds sterilization.

Moist Heat: - There are two types of moist heat i.e. Boiling and Autoclaving.

1. Boiling: - Although effective in killing non sporing bacteria in a short-time is often relatively in effective against bacterial spores but it is generally safe because the instrument are clean before boiling and spores are rare under such circumstances. It is also used for minor rather than major surgical procedures.
For example: - Coagulation of protein
Take a beaker which is filled with water, boil the water at 100*c and water converts into the steam. When we applied the pressure the steam converts into the liquid water and the temperature increases. Now put the thing in a beaker at high temperature for sterilization.